The most
common way of measuring nitrogen fixation is by an indirect method which uses
the ability of the nitrogenase enzyme to reduce triple bounded substrates,
estimates nitrogenase activity by measuring the rate of acetylene reduced to
ethylene. Both gases can easily be quantified using gas chromatography. This
method is called acetylene reduction assay. The main benefit of acetylene
reduction assay is that it is easy to use, sensitive and has a good
reproducibility.
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| Acetylene reduction assay |
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