Friday, 22 March 2013

Acetylene reduction assay


The most common way of measuring nitrogen fixation is by an indirect method which uses the ability of the nitrogenase enzyme to reduce triple bounded substrates, estimates nitrogenase activity by measuring the rate of acetylene reduced to ethylene. Both gases can easily be quantified using gas chromatography. This method is called acetylene reduction assay. The main benefit of acetylene reduction assay is that it is easy to use, sensitive and has a good reproducibility.
Acetylene reduction assay

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